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2012, Sensors
Materials Science and Engineering: C
Entrapment of enzyme within organic and inorganic materials for biosensor applications: Comparative study2006 •
ABSTRACT In order to determine the best kind of matrix that enables preservation of the enzymatic and electro-enzymatic activity of immobilized enzyme and provides accessibility towards substrate, various host materials (electropolymerized polypyrrole films, alginate polysaccharide, biocompatible synthetic latex and inorganic clays (laponite and layered double hydroxides LDHs), differing in permeability, ion exchange properties and hydrophobic–hydrophilic character, were compared for the fabrication of amperometric glucose biosensors. The electrochemical assays were performed by potentiostating the enzyme electrodes at 0.6 V vs. Ag/AgCl in order to oxidize the hydrogen peroxide enzymatically generated in the presence of glucose and oxygen. The highest sensitivity and maximum current density were recorded for laponite (82.3 mA M−1 cm−2 and 410 μA cm−2 respectively) and LDHs (55 mA M−1 cm−2 and 417 μA cm−2, respectively).
Analytical and Bioanalytical Chemistry
Development of electrochemical biosensors based on sol-gel enzyme encapsulation and protective polymer membranes2008 •
The electrochemical biosensor with enzyme as biorecognition element is traditionally pursued as an attractive research topic owing to their high commercial perspective in healthcare and environmental sectors. The research interest on the subject is sharply increased since the beginning of 21st century primarily, due to the concomitant increase in knowledge in the field of material science. The remarkable effects of many advance materials such as, conductive polymers and nanomaterials, were acknowledged in the developing efficient 3rd generation enzyme bioelectrodes which offer superior selectivity, sensitivity, reagent less detection, and label free fabrication of biosensors. The present review article compiles the major knowledge surfaced on the subject since its inception incorporating the key review and experimental papers published during the last decade which extensively cover the development on the redox enzyme based 3rd generation electrochemical biosensors. The tenet involved in the function of these direct electrochemistry based enzyme electrodes, their characterizations and various strategies reported so far for their development such as, nanofabrication, polymer based and reconstitution approaches are elucidated. In addition, the possible challenges and the future prospects in the development of efficient biosensors following this direct electrochemistry based principle are discussed. A comparative account on the design strategies and critical performance factors involved in the 3rd generation biosensors among some selected prominent works published on the subject during last decade have also been included in a tabular form for ready reference to the readers.
Biosensors - Micro and Nanoscale Applications
New Materials for the Construction of Electrochemical Biosensors2015 •
Kocaeli Journal of Science and Engineering
Conducting Polymer Based Enzyme Electrodes Fabricated by Invertase and Polyphenol Oxidase2021 •
Novel carbon fiber enzyme electrodes were constructed and evaluated. Fabrication of electrodes was performed via electrochemical deposition of a conducting matrice composed of polythiophene and polypyrrole (PTh–PPy) onto carbon fiber substrates. The enzyme was entrapped into the matrix during electropolymerization. Resultant biosensors represented higher kinetic parameters, Vmax and Km, in comparison to PPy matrice, which are 2.471 ± 0.150 mol min1- electrode1- and 30.60 ± 5.30 mM for invertase, 0.056 ± 0.012 mol min1- electrode1- and 842.00 ± 37.50 mM for polyphenol oxidase respectively. Optimum pH and temperature of the immobilized enzyme within PTh–PPy composite indicates that this matrice provides a more protective environment. The detection limit (LOD) of polyphenols was obtained as 0.037 mg mL1-. Polyphenol oxidase enzyme electrodes were proved to be used for the determination of polyphenolic substances in real samples and the results were confirmed by the Folin-Ciocalteau m...
Nanoscale Advances
Advances in nanomaterial application in enzyme-based electrochemical biosensors: a review2019 •
Application of nanomaterials greatly improves performance of electrochemical enzyme-based biosensors.
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